Digital Holographic Microscopy: A Quantitative Label-Free Microscopy Technique for Phenotypic Screening

Digital Holographic Microscopy: A Quantitative Label-Free Microscopy Technique for Phenotypic Screening

Digital Holographic Microscopy (DHM) is a label-free imaging technique allowing visualization of transparent cells with classical imaging cell culture plates. The quantitative DHM phase contrast image provided is related both to the intracellular refractive index and to cell thickness. DHM is able to distinguish cellular morphological changes on two representative cell lines (HeLa and H9c2) whe...

Digital in-line holographic microscopy.

We first briefly review the state of the art of digital in-line holographic microscopy (DIHM) with numerical reconstruction and then discuss some technical issues, such as lateral and depth resolution, depth of field, twin image, four-dimensional tracking, and reconstruction algorithm. We then present a host of examples from microfluidics and biology of tracking the motion of spheres, algae, an...

Speckle-field digital holographic microscopy.

The use of coherent light in conventional holographic phase microscopy (HPM) poses three major drawbacks: poor spatial resolution, weak depth sectioning, and fixed pattern noise due to unwanted diffraction. Here, we report a technique which can overcome these drawbacks, but maintains the advantage of phase microscopy - high contrast live cell imaging and 3D imaging. A speckle beam of a complex ...

Digital In-line Holographic Microscopy

Three-dimensional imaging with micron resolution and simultaneous tracking of many (hundreds) objects (bubbles, beads, algae, bacteria etc.) is now possiblein situ not only with a benchtop microscope, but also remotely in such environments as the deep ocean with a submersible version of the instrument. The instrument is very simple in its hardware requirements and an efficient software package ...

Quantitative Analysis of Biological Cells Using Digital Holographic Microscopy